CRISPR Guide Designer
Design optimal guide RNAs for CRISPR-Cas9 genome editing. Detect PAM sites, score guides, and predict off-target effects — all in your browser.
PAM Site Detection
Finds NGG and custom PAM motifs in both DNA strands.
Guide Scoring
Scores guides 0-100 based on GC content, Tm, and structure.
Off-Target Analysis
Detects potential off-target issues and flags warnings.
RNA Conversion
Displays the guide RNA sequence (T→U conversion).
Strand Detection
Finds guides on both forward and reverse strands.
Export & Save
Save guides with full analysis for future reference.